Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney
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چکیده
منابع مشابه
Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney.
A fluorescein-labelled antiserum to human Tamm-Horsfall mucoprotein applied to frozen human kidney sections gave strong specific labelling, mainly of cells of tubules in the outer medulla. By comparison with adjacent serial sections stained for alkaline phosphatase and succinic dehydrogenase, it is suggested that material reacting immunologically as Tamm-Horsfall muco-protein is found particula...
متن کاملProperties of a glycopeptide isolated from human Tamm-Horsfall glycoprotein. Interaction with leucoagglutinin and anti-(human Tamm-Horsfall glycoprotein) antibodies.
A sialylated glycopeptide isolated after Pronase digestion of human Tamm-Horsfall glycoprotein behaves as a powerful monovalent hapten in the precipitin reaction between human Tamm-Horsfall glycoprotein and leucoagglutinin, but fails to inhibit the interaction of the glycoprotein with rabbit anti-(human Tamm-Horsfall glycoprotein) antibodies. The glycopeptide is much less active than the intact...
متن کاملLocalization of a single binding site for immunoglobulin light chains on human Tamm-Horsfall glycoprotein.
Cast nephropathy is a severe complication of multiple myeloma. Binding of filtered monoclonal light chains (LC) with Tamm-Horsfall glycoprotein (THP) triggers heterotypic aggregation of these two proteins to form casts in the distal nephron of the kidney. To localize the LC binding site on THP, human THP was deglycosylated and underwent limited trypsin digestion in the presence or absence of a ...
متن کاملFactors affecting excretion of human urinary Tamm-Horsfall glycoprotein.
1. Tamm-Horsfall glycoprotein was determined, by radioimmunoassay, in samples of urine from normal individuals under a variety of physiological conditions. 2. The amount of glycoprotein excreted in 24 h by our population (39 +/- 13 mg, corrected for body surface area) was found not to be influenced by sex, age (19-60 years) or amounts of Ca2+, Mg2+ and Na+ excreted. 3. Urine samples collected a...
متن کاملHigh-mannose oligosaccharides from human Tamm-Horsfall glycoprotein.
The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-beta-N-acetylglucosaminidase H, fo...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1969
ISSN: 0021-9746
DOI: 10.1136/jcp.22.3.334